Abstract

Background: Currently questions on the role of genes polymorphism that regulate metabolism and implicated in metabolic disorders are disputed. Objective. The study of proteomic and metabolomic manifestations of different variants of apolipoprotein E genes polymorphism in obese people.     

Methods: The study involved 217 obese people.All the patients underwent genotyping of polymorphic sites с.388 (T>C) c.526 (C>T) of the ApoE gene. Proteomic studies included the determination of the content ofadipokines (adiponectin, resistin, apelin and visfatin), cytokines (IL-6, TNFa, IL-1a), CRP and fatty acids L-FABP, annexin V. Assessment of metabolomic markers was based on a study of the lipid profile. Results: It was established that, with the ApoE genotypes ε3 / ε3 and ε2 / ε3 of the ApoE gene, the content of lipids and glucose in patients with obesity was statistically significantly reduced, which indicated a high efficiency of diet therapy. In patients with the genotypes ε2 / ε2 and ε4 / ε3, no such patterns were found. The most pronounced changes in lipid metabolism are revealed in carriers of C allele at position c.388 and T at position c.526 of ApoE gene (c.388 T/C+c.526C/T and c.388 T/C+c.526 T/T). In the course of the execution of the correlation analysis of metabolomic and proteomic markers 1SNP or absence of SNP (c.388 T/T+c.526 C/T or s.388 T/C+c.526 C/C) in ApoE gene was found to be accompanied with dyslipidemia and the initiation of inflammation. At 2 SNP in the ApoE gene (c.388 T/T+c.526 T/T and c.388 T/C+c.526 C/T), we have identified correlations of lipid metabolism markers and adipokines (adiponectin, visfatin). At 3 SNP we found the relationship between adipokines (apelin, visfatin, resistin) and pro-inflammatory cytokines (IL1a), annexin V and lipid metabolism markers.

Conclusion: The studied polymorphic genotypes may be used in the complex with the individual characteristics of energy metabolism, hormonal and immune status of the people for preventive medical measures and the implementation of a personalized diet of obese people..